normal human endometrial epithelial cells heec  (ATCC)

Journal: ACS Omega

Article Title: Bioinformatics Analysis and Experimental Verification Define Different Angiogenesis Subtypes in Endometrial Carcinoma and Identify a Prognostic Signature

doi: 10.1021/acsomega.4c03034

Figure Lengend Snippet: Expression levels of selected angiogenesis-related hub genes. (A) Differential transcription levels of IHH, TUBG1, EEF2, and MECP2 in UCEC cell lines and hEECs. (B) Violin diagrams of differential transcript levels of IHH, TUBG1, EEF2, and MECP2 in UCEC tissues and paratumor tissues. (C) Comparison of the protein expression levels of IHH, TUBG1, EEF2, and MECP2 between UCEC and paratumor by immunohistochemistry staining and analyzed by calculating average optical density (AOD: IOD/area). (D) Representative immunohistochemistry staining images. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns no significance.

Article Snippet: Human endometrial cancer cell lines (RL-952, KLE, HEC-1B, HEC-1A, and Ishikawa), normal human endometrial epithelial cells (hEECs), and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Comparison, Immunohistochemistry, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Eukaryotic initiation factor 3B downregulation suppresses cell proliferation, migration and invasion while it induces cell apoptosis by blocking the β-catenin pathway in endometrial cancer

doi:

Figure Lengend Snippet: EIF3B expression in human endometrial cancer cells and normal human endometrial (uterine) epithelial cells. Comparison of EIF3B mRNA expression between human endometrial cancer cells (Ishikawa, HEC-1A, RL95-2 and EFE-184 cell lines) and normal human endometrial (uterine) epithelial cells (HEEC cell line) (A). EIF3B protein expression in human endometrial cancer cells and normal human endometrial (uterine) epithelial cells (B). Comparisons among groups were detected using one-way ANOVA followed by Tukey’s multiple comparison test. A P value < 0.05 was considered significant. *P < 0.05, ***P < 0.001. EIF3B, eukaryotic initiation factor 3B; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, not significant.

Article Snippet: In addition, normal human endometrial (uterine) epithelial cell line (HEEC) was purchased from Lifeline® Cell Technology (CA, USA).

Techniques: Expressing, Comparison